BAIT

CIN8

KSL2, SDS15, kinesin motor protein CIN8, L000000340, YEL061C
Kinesin motor protein; involved in mitotic spindle assembly and chromosome segregation
Saccharomyces cerevisiae (S288c)
PREY

SHP1

UBX1, protein phosphatase regulator SHP1, L000002746, YBL058W
UBX (ubiquitin regulatory X) domain-containing protein; regulates Glc7p phosphatase activity; shp1 mutants are impaired in growth and mitotic progression; functions in growth and mitotic progression require Cdc48p binding; mitotic phenotype is caused by reduced Glc7p activity; interacts with ubiquitylated proteins, required for degradation of a ubiquitylated model substrate
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A robust toolkit for functional profiling of the yeast genome.

Pan X, Yuan DS, Xiang D, Wang X, Sookhai-Mahadeo S, Bader JS, Hieter P, Spencer F, Boeke JD

Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAGs and the SGA reporter, MFA1pr-HIS3, which facilitates conversion of heterozygous diploid YKO strains into haploid ... [more]

Mol. Cell Nov. 05, 2004; 16(3);487-96 [Pubmed: 15525520]

Throughput

  • High Throughput

Ontology Terms

  • inviable (APO:0000112)

Curated By

  • BioGRID