Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

termination of RNA polymerase II transcription [IMP]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

core mediator complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A robust toolkit for functional profiling of the yeast genome.

Pan X, Yuan DS, Xiang D, Wang X, Sookhai-Mahadeo S, Bader JS, Hieter P, Spencer F, Boeke JD

Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAGs and the SGA reporter, MFA1pr-HIS3, which facilitates conversion of heterozygous diploid YKO strains into haploid ... [more]

Mol. Cell Nov. 05, 2004; 16(3);487-96 [Pubmed: 15525520]

Throughput

High Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Curated By

BioGRID

Interaction Summary

CIN8

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule motor activity [IGI]minus-end-directed microtubule motor activity [IDA]plus-end-directed microtubule motor activity [IDA]

Gene Ontology Cellular Component

SRB5

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]core RNA polymerase II binding transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

A robust toolkit for functional profiling of the yeast genome.

Throughput

Ontology Terms

Curated By

microtubule motor activity [IGI]
minus-end-directed microtubule motor activity [IDA]
plus-end-directed microtubule motor activity [IDA]

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
core RNA polymerase II binding transcription factor activity [IDA, IGI]