Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication

GO Process (3)

GO Function (1)

GO Component (4)

Gene Ontology Biological Process

nuclear pore organization [IGI]

protein import into nucleus [IGI]

spindle pole body duplication [IGI]

Gene Ontology Molecular Function

structural constituent of nuclear pore [IC]

Gene Ontology Cellular Component

integral component of membrane [ISM]

integral component of nuclear outer membrane [IDA]

nuclear pore [IDA]

nuclear pore transmembrane ring [IDA, IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation.

Menon BB, Sarma NJ, Pasula S, Deminoff SJ, Willis KA, Barbara KE, Andrews B, Santangelo GM

The recruitment model for gene activation presumes that DNA is a platform on which the requisite components of the transcriptional machinery are assembled. In contrast to this idea, we show here that Rap1/Gcr1/Gcr2 transcriptional activation in yeast cells occurs through a large anchored protein platform, the Nup84 nuclear pore subcomplex. Surprisingly, Nup84 and associated subcomplex components activate transcription themselves in ... [more]

Proc. Natl. Acad. Sci. U.S.A. Apr. 19, 2005; 102(16);5749-54 [Pubmed: 15817685]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GCR1 POM34	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Menon BB (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GCR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]

Gene Ontology Cellular Component

POM34

Gene Ontology Biological Process

Gene Ontology Molecular Function

structural constituent of nuclear pore [IC]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Reverse recruitment: the Nup84 nuclear pore subcomplex mediates Rap1/Gcr1/Gcr2 transcriptional activation.

Throughput

Ontology Terms

Related interactions

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA, IMP]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]