BAIT

MAD1

coiled-coil domain-containing protein MAD1, L000000974, YGL086W

Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability

GO Process (5)

GO Function (0)

GO Component (3)

Gene Ontology Biological Process

activation of mitotic cell cycle spindle assembly checkpoint [IMP]

mitotic DNA integrity checkpoint [IGI]

mitotic spindle assembly checkpoint [IGI, IMP]

negative regulation of protein import into nucleus during spindle assembly checkpoint [IMP]

nucleocytoplasmic transport [IGI, IPI]

Gene Ontology Cellular Component

kinetochore [IDA, IMP]

nuclear pore [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CIN1

L000000336, YOR349W

Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl

GO Process (3)

GO Function (1)

GO Component (0)

Gene Ontology Biological Process

protein folding [IGI, ISS]

tubulin complex assembly [IGI]

tubulin complex biogenesis [IMP]

Gene Ontology Molecular Function

beta-tubulin binding [ISS]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.

Daniel JA, Keyes BE, Ng YP, Freeman CO, Burke DJ

The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis ... [more]

Genetics Jan. 01, 2006; 172(1);53-65 [Pubmed: 16157669]

Throughput

High Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CIN1 MAD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-9.5056	BioGRID	214420
MAD1 CIN1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3744	BioGRID	380436
CIN1 MAD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3744	BioGRID	417892
CIN1 MAD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.5517	BioGRID	2187577
MAD1 CIN1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.4725	BioGRID	2115310
MAD1 CIN1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Kuzmin E (2018)	High	-0.675	BioGRID	2429693
CIN1 MAD1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Zheng J (2010)	High	-10.537	BioGRID	508479
MAD1 CIN1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Lee MS (2004)	Low	-	BioGRID	165992
CIN1 MAD1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	113052

Curated By

BioGRID

Interaction Summary

MAD1

Gene Ontology Biological Process

Gene Ontology Cellular Component

CIN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-tubulin binding [ISS]

Synthetic Lethality

Publication

Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.

Throughput

Ontology Terms

Related interactions

Curated By