BAIT
MAD1
coiled-coil domain-containing protein MAD1, L000000974, YGL086W
Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
GO Process (5)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Cellular Component
- kinetochore [IDA, IMP]
- nuclear pore [IDA]
- nucleus [IDA]
Saccharomyces cerevisiae (S288c)
PREY
SPT4
transcription elongation factor SPT4, L000002030, YGR063C
Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions
GO Process (12)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [IGI]
- chromatin organization [IMP]
- chromatin silencing [IMP]
- chromosome segregation [IMP]
- intracellular mRNA localization [IMP]
- mRNA splicing, via spliceosome [IMP]
- negative regulation of transcription elongation from RNA polymerase I promoter [IGI]
- positive regulation of transcription elongation from RNA polymerase I promoter [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- regulation of rRNA processing [IMP]
- regulation of transcription, DNA-templated [IMP]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.
The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis ... [more]
Genetics Jan. 01, 2006; 172(1);53-65 [Pubmed: 16157669]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID