BAIT
SPT3
transcriptional regulator SPT3, L000002029, YDR392W
Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; relocalizes to the cytosol in response to hypoxia
GO Process (5)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
PAF1
L000002621, YBR279W
Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
GO Process (25)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- chromatin organization involved in regulation of transcription [IMP]
- chromatin silencing at rDNA [IMP]
- global genome nucleotide-excision repair [IMP]
- mRNA 3'-end processing [IMP]
- negative regulation of DNA recombination [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of histone H3-K36 trimethylation [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- rRNA processing [IMP]
- regulation of chromatin silencing at telomere [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA]
- regulation of histone H2B ubiquitination [IMP]
- regulation of histone H3-K4 methylation [IMP]
- regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- snoRNA 3'-end processing [IMP]
- snoRNA transcription from an RNA polymerase II promoter [IDA, IMP]
- transcription elongation from RNA polymerase I promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
- transcription from RNA polymerase I promoter [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
H2B ubiquitin protease Ubp8 and Sgf11 constitute a discrete functional module within the Saccharomyces cerevisiae SAGA complex.
The SAGA complex is a multisubunit protein complex involved in transcriptional regulation in Saccharomyces cerevisiae. SAGA combines proteins involved in interactions with DNA-bound activators and TATA-binding protein (TBP), as well as enzymes for histone acetylation (Gcn5) and histone deubiquitylation (Ubp8). We recently showed that H2B ubiquitylation and Ubp8-mediated deubiquitylation are both required for transcriptional activation. For this study, we investigated ... [more]
Mol. Cell. Biol. Feb. 01, 2005; 25(3);1162-72 [Pubmed: 15657441]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID