Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The familial mediterranean fever protein interacts and colocalizes with a putative Golgi transporter.

Chen X, Bykhovskaya Y, Tidow N, Hamon M, Bercovitz Z, Spirina O, Fischel-Ghodsian N

The biological function of pyrin, the protein mutated in Familial Mediterranean Fever (FMF), has not been elucidated. Based on sequence homology, a transcription factor activity was proposed for this neutrophil-specific protein. In a yeast two-hybrid assay, neither transcription activation activity nor any self interaction was detected for pyrin. Screening of an expression cDNA library of peripheral blood leukocytes using as ... [more]

Proc. Soc. Exp. Biol. Med. May. 01, 2000; 224(1);32-40 [Pubmed: 10782044]

Throughput

  • Low Throughput

Curated By

  • BioGRID