Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human Krueppel-like factor 8: a CACCC-box binding protein that associates with CtBP and represses transcription.

van Vliet J, Turner J, Crossley M

CACCC-boxes are recognised by transcription factors of the Sp/Krueppel-like Factor (Sp1/KLF) family. Here we describe one member of this family, KLF8/ZNF741/BKLF3 (KLF8). KLF8 contains a characteristic C-terminal DNA-binding domain comprised of three Krueppel-like zinc fingers, but also has limited homology to another family member, KLF3/Basic Krueppel-like Factor (KLF3/BKLF), in its N-terminus. Most significantly, it shares with KLF3/BKLF a Pro-Val-Asp-Leu-Ser/Thr motif. ... [more]

Nucleic Acids Res. May. 01, 2000; 28(9);1955-62 [Pubmed: 10756197]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CTBP2 KLF8
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID