Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis.

Yuryev A, Wennogle LP

We have performed an exhaustive unbiased yeast two-hybrid analysis to identify interaction partners of two human Raf kinase isoforms, A-Raf and C-Raf, using their N-terminal regulatory domain as "bait." A total of 20 different human proteins were found to interact with Raf isoforms. Several of these interactions were novel and an extensive bioinformatics evaluation was performed for each. The novel ... [more]

Genomics Feb. 01, 2003; 81(2);112-25 [Pubmed: 12620389]


  • High Throughput

Curated By

  • BioGRID