IES5
Gene Ontology Biological Process
Gene Ontology Cellular Component
TAF14
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IC]
- chromatin remodeling [IC]
- histone acetylation [IDA]
- nucleosome mobilization [IDA]
- promoter clearance from RNA polymerase II promoter [IC]
- transcription elongation from RNA polymerase II promoter [IC]
- transcription from RNA polymerase II promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA]
- transcriptional start site selection at RNA polymerase II promoter [IC]
Gene Ontology Molecular Function- DNA translocase activity [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
- core RNA polymerase II binding transcription factor activity [IDA]
- core RNA polymerase II recruiting transcription factor activity [IC]
- DNA translocase activity [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
- core RNA polymerase II binding transcription factor activity [IDA]
- core RNA polymerase II recruiting transcription factor activity [IC]
Gene Ontology Cellular Component
- Ino80 complex [IDA, IPI]
- NuA3 histone acetyltransferase complex [IDA]
- NuA3a histone acetyltransferase complex [IDA]
- NuA3b histone acetyltransferase complex [IDA]
- SWI/SNF complex [IDA, IPI]
- mediator complex [IPI]
- nucleus [IDA]
- transcription factor TFIID complex [IDA]
- transcription factor TFIIF complex [IDA, IPI]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteome survey reveals modularity of the yeast cell machinery.
Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. Here we report the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry. Through systematic tagging of open reading frames (ORFs), the majority of complexes were purified several times, suggesting screen saturation. The richness of the data set ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IES5 TAF14 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 10 | BioGRID | 3618183 | |
| TAF14 IES5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| TAF14 IES5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| TAF14 IES5 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -7.2757 | BioGRID | 215517 |
Curated By
- BioGRID