Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human fatty acid synthase: role of interdomain in the formation of catalytically active synthase dimer.

Chirala SS, Jayakumar A, Gu ZW, Wakil SJ

The human and animal fatty acid synthases are dimers of two identical multifunctional proteins (M(r) 272,000) arranged in an antiparallel configuration. This arrangement generates two active centers for fatty acid synthesis separated by interdomain (ID) regions and predicts that two appropriate halves of the monomer should be able to reconstitute an active fatty acid synthesizing center. This prediction was confirmed ... [more]

Proc. Natl. Acad. Sci. U.S.A. Mar. 13, 2001; 98(6);3104-8 [Pubmed: 11248039]

Throughput

  • Low Throughput

Curated By

  • BioGRID