Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The C terminus of mitosin is essential for its nuclear localization, centromere/kinetochore targeting, and dimerization.

Zhu X, Chang KH, He D, Mancini MA, Brinkley WR, Lee WH

Mitosin is a novel 350-kDa nuclear phosphoprotein that dramatically relocates from the evenly nuclear distribution in S phase to the centromere/kinetochore and mitotic apparatus in M phase. The dynamic relocalization of mitosin is accompanied by the phosphorylation of itself, suggesting that mitosin plays a role in mitotic progression. The molecular basis of nuclear localization and targeting of mitosin to the ... [more]

J. Biol. Chem. Aug. 18, 1995; 270(33);19545-50 [Pubmed: 7642639]

Throughput

  • Low Throughput

Curated By

  • BioGRID