NSE3
Gene Ontology Biological Process
- DNA repair [IMP, IPI]
Gene Ontology Molecular Function
NSE4
Gene Ontology Biological Process
- DNA repair [IMP, IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global genetic interaction network maps a wiring diagram of cellular function.
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]
Quantitative Score
- -0.5941 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
- alleles: nse3-ts3 - nse4-5001 [SGA score = -0.4821, P-value = 6.573E-156]
- alleles: nse3-ts3 - nse4-ts1 [SGA score = -0.4710, P-value = 0]
- alleles: nse3-ts3 - nse4-ts2 [SGA score = -0.3469, P-value = 1.528E-30]
- alleles: nse3-ts3 - nse4-ts3 [SGA score = -0.4845, P-value = 1.063E-67]
- alleles: nse3-ts3 - nse4-ts4 [SGA score = -0.3688, P-value = 0]
- alleles: nse3-ts4 - nse4-5001 [SGA score = -0.5941, P-value = 1.144E-89]
- alleles: nse3-ts4 - nse4-ts1 [SGA score = -0.5561, P-value = 2.586E-69]
- alleles: nse3-ts4 - nse4-ts2 [SGA score = -0.4272, P-value = 9.639E-56]
- alleles: nse3-ts4 - nse4-ts3 [SGA score = -0.5773, P-value = 6.127E-141]
- alleles: nse3-ts4 - nse4-ts4 [SGA score = -0.5053, P-value = 1.303E-128]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NSE3 NSE4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| NSE4 NSE3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| NSE4 NSE3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 6 | BioGRID | 3612507 | |
| NSE4 NSE3 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
| NSE4 NSE3 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 163816 | |
| NSE4 NSE3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.6344 | BioGRID | 1923590 | |
| NSE3 NSE4 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| NSE3 NSE4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NSE4 NSE3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NSE3 NSE4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| NSE4 NSE3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NSE3 NSE4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID