BAIT

NDN

HsT16328, PWCR
necdin, melanoma antigen (MAGE) family member
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens
PREY

NUBP1

NBP, NBP1, NBP35
nucleotide binding protein 1
GO Process (4)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm.

Taniguchi N, Taniura H, Niinobe M, Takayama C, Tominaga-Yoshino K, Ogura A, Yoshikawa K

Necdin, a growth suppressor expressed predominantly in postmitotic neurons, interacts with viral oncoproteins and cellular transcription factors E2F1 and p53. In search of other cellular targets of necdin, we screened cDNA libraries from neurally differentiated murine embryonal carcinoma P19 cells and adult rat brain by the yeast two-hybrid assay. We isolated cDNAs encoding partial sequences of mouse NEFA and rat ... [more]

J. Biol. Chem. Oct. 13, 2000; 275(41);31674-81 [Pubmed: 10915798]

Throughput

  • Low Throughput

Curated By

  • BioGRID