PREY

ARP2

ACT2, actin-related protein 2, L000000026, YDL029W
Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

  • -0.5914 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
  • alleles: act1-101 - arp2-14 [SGA score = -0.5914, P-value = 1.796E-48]
  • alleles: act1-108 - arp2-14 [SGA score = -0.4740, P-value = 1.58E-14]
  • alleles: act1-119 - arp2-14 [SGA score = -0.3946, P-value = 5.202E-8]
  • alleles: act1-120 - arp2-14 [SGA score = -0.3279, P-value = 0]
  • alleles: act1-121 - arp2-14 [SGA score = -0.3393, P-value = 1.78E-7]
  • alleles: act1-124 - arp2-14 [SGA score = -0.4957, P-value = 2.539E-12]
  • alleles: act1-125 - arp2-14 [SGA score = -0.4414, P-value = 3.788E-20]
  • alleles: act1-129 - arp2-14 [SGA score = -0.2795, P-value = 0.02748]
  • alleles: act1-132 - arp2-14 [SGA score = -0.2883, P-value = 5.935E-5]
  • alleles: act1-133 - arp2-14 [SGA score = -0.2356, P-value = 8.091E-5]
  • alleles: act1-136 - arp2-14 [SGA score = -0.4979, P-value = 0]
  • alleles: act1-159 - arp2-14 [SGA score = -0.1573, P-value = 0.02703]
  • alleles: act1-3 - arp2-14 [SGA score = -0.5562, P-value = 1.243E-75]
  • alleles: act1-4 - arp2-14 [SGA score = -0.4398, P-value = 4.219E-18]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARP2 ACT1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.6238BioGRID
1922994
ARP2 ACT1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
ACT1 ARP2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
ARP2 ACT1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
ACT1 ARP2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID