BAIT

NOP9

YJL010C
Essential subunit of U3-containing 90S preribosome; involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
Saccharomyces cerevisiae (S288c)
PREY

ACT1

ABY1, END7, actin, L000000025, YFL039C
Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

  • -0.3404 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
  • alleles: nop9-5001 - act1-119 [SGA score = -0.1239, P-value = 7.804E-7]
  • alleles: nop9-5001 - act1-120 [SGA score = -0.2832, P-value = 2.638E-32]
  • alleles: nop9-5001 - act1-122 [SGA score = -0.1978, P-value = 2.705E-7]
  • alleles: nop9-5001 - act1-124 [SGA score = -0.2313, P-value = 1.045E-27]
  • alleles: nop9-5001 - act1-125 [SGA score = -0.2815, P-value = 0]
  • alleles: nop9-5001 - act1-129 [SGA score = -0.3404, P-value = 9.25E-13]
  • alleles: nop9-5001 - act1-136 [SGA score = -0.1283, P-value = 0.007529]
  • alleles: nop9-5001 - act1-155 [SGA score = -0.2988, P-value = 8.73E-18]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ACT1 NOP9
Synthetic Haploinsufficiency
Synthetic Haploinsufficiency

A genetic interaction is inferred when mutations or deletions in separate genes, at least one of which is hemizygous, cause a minimal phenotype alone but result in lethality when combined in the same cell under a given condition.

Low/High-BioGRID
572771

Curated By

  • BioGRID