BAIT

RVS161

END6, FUS7, SPE161, amphiphysin-like protein RVS161, L000001788, YCR009C

Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress

GO Process (8)

GO Function (2)

GO Component (5)

Gene Ontology Biological Process

actin cortical patch localization [IMP]

actin cytoskeleton organization [IMP]

actin cytoskeleton reorganization [IMP]

conjugation with cellular fusion [IMP]

endocytosis [IMP]

lipid tube assembly [IDA]

response to osmotic stress [IMP]

response to starvation [IMP]

Gene Ontology Molecular Function

cytoskeletal protein binding [IPI]
lipid binding [IDA]

Gene Ontology Cellular Component

Rvs161p-Rvs167p complex [IPI]

actin cortical patch [IDA]

mating projection [IDA]

mating projection tip [IDA]

membrane raft [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Characterization of the yeast amphiphysins Rvs161p and Rvs167p reveals roles for the Rvs heterodimer in vivo.

Friesen H, Humphries C, Ho Y, Schub O, Colwill K, Andrews B

We have used comprehensive synthetic lethal screens and biochemical assays to examine the biological role of the yeast amphiphysin homologues Rvs161p and Rvs167p, two proteins that play a role in regulation of the actin cytoskeleton, endocytosis, and sporulation. We found that unlike some forms of amphiphysin, Rvs161p-Rvs167p acts as an obligate heterodimer during vegetative growth and neither Rvs161p nor Rvs167p ... [more]

Mol. Biol. Cell Mar. 01, 2006; 17(3);1306-21 [Pubmed: 16394103]

Throughput

High Throughput|Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Additional Notes

High Throughput: Synthetic Genetic Array (SGA) analysis
Low Throughput: Confirmed by tetrad analysis.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SWI4 RVS161	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Collins SR (2007)	High	-6.2745	BioGRID	216271
SWI4 RVS161	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.1812	BioGRID	375818
SWI4 RVS161	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.224	BioGRID	2109520
RVS161 SWI4	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	112870

Curated By

BioGRID

Interaction Summary

RVS161

Gene Ontology Biological Process

Gene Ontology Molecular Function

cytoskeletal protein binding [IPI]lipid binding [IDA]

Gene Ontology Cellular Component

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Synthetic Lethality

Publication

Characterization of the yeast amphiphysins Rvs161p and Rvs167p reveals roles for the Rvs heterodimer in vivo.

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

cytoskeletal protein binding [IPI]
lipid binding [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]