PMF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NFE2L2
Gene Ontology Biological Process
- cellular response to fluid shear stress [IDA]
- cellular response to hydrogen peroxide [IMP]
- cellular response to laminar fluid shear stress [IMP]
- cellular response to tumor necrosis factor [IMP]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of hydrogen peroxide-induced cell death [IGI]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IMP]
- positive regulation of gene expression [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to stress [IMP]
- proteasomal ubiquitin-independent protein catabolic process [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein ubiquitination [IDA]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [TAS]
- transcription regulatory region sequence-specific DNA binding [TAS]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Cloning and characterization of human polyamine-modulated factor-1, a transcriptional cofactor that regulates the transcription of the spermidine/spermine N(1)-acetyltransferase gene.
The increased transcription and ultimate superinduction of the spermidine/spermine N(1)-acetyltransferase (SSAT) gene has been associated with the antineoplastic activity of several new antitumor polyamine analogues. In sensitive tumor cell types, the transcriptional induction appears to be regulated by the constitutive association of the transcription factor Nrf-2 with the recently discovered polyamine-responsive element. Using the yeast two-hybrid system, a new transcriptional ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NFE2L2 PMF1 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 3012631 | |
| PMF1 NFE2L2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID