BAIT

MED11

BcDNA:GH01072, CG6884, Dmel\CG6884, Med21, anon-WO0153538.71, dMED21, Dmel_CG6884
Mediator complex subunit 11
Drosophila melanogaster

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Novel Mediator proteins of the small Mediator complex in Drosophila SL2 cells.

Gu JY, Park JM, Song EJ, Mizuguchi G, Yoon JH, Kim-Ha J, Lee KJ, Kim YJ

The Mediator complex is generally required for transcriptional regulation in species ranging from yeast to human. Throughout evolution, the functional diversity of the Mediator complex has been enhanced to meet the increasing requirements for sophisticated gene regulation. It is likely that greater structural complexity is thus required to accomplish these new, complex regulatory functions. In this study, we took systematic ... [more]

J. Biol. Chem. Jul. 26, 2002; 277(30);27154-61 [Pubmed: 12021283]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MED6 MED11
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID