NCK1
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- T cell activation [IMP]
- T cell receptor signaling pathway [TAS]
- axon guidance [TAS]
- innate immune response [TAS]
- negative regulation of cell death [IDA]
- negative regulation of protein kinase activity [IDA]
- positive regulation of T cell proliferation [IMP]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- signal complex assembly [NAS]
Gene Ontology Molecular Function
EPHB1
Gene Ontology Biological Process
- angiogenesis [IDA]
- axon guidance [ISS]
- cell chemotaxis [IDA]
- cell-substrate adhesion [IDA]
- central nervous system projection neuron axonogenesis [ISS]
- dendritic spine development [ISS]
- dendritic spine morphogenesis [ISS]
- detection of temperature stimulus involved in sensory perception of pain [ISS]
- ephrin receptor signaling pathway [IDA]
- establishment of cell polarity [ISS]
- neural precursor cell proliferation [ISS]
- neurogenesis [ISS]
- positive regulation of synapse assembly [ISS]
- protein autophosphorylation [IDA]
- regulation of ERK1 and ERK2 cascade [IDA]
- regulation of JNK cascade [IDA]
- regulation of neuron death [IMP]
- retinal ganglion cell axon guidance [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase.
Eph family receptor tyrosine kinases signal axonal guidance, neuronal bundling, and angiogenesis; yet the signaling systems that couple these receptors to targeting and cell-cell assembly responses are incompletely defined. Functional links to regulators of cytoskeletal structure are anticipated based on receptor mediated cell-cell aggregation and migratory responses. We used two-hybrid interaction cloning to identify EphB1-interactive proteins. Six independent cDNAs encoding ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| EPHB1 NCK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID