Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL.

Seki T, Tada S, Katada T, Enomoto T

We isolated two cDNA clones encoding human proteins which interact with DNA helicase Q1/RecQL, a human homologue of Eschelichia coli RecQ protein, by two-hybrid screening. One of these proteins, named Qip1, was a novel protein homologous to the nuclear localization signal (NLS) receptor importin-alpha, and the other was the known protein Rch1, which is also a homologue of importin-alpha. DNA ... [more]

Biochem. Biophys. Res. Commun. May. 08, 1997; 234(1);48-53 [Pubmed: 9168958]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RECQL KPNA4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Seki T (1997)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RECQL

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP-dependent DNA helicase activity [TAS]DNA helicase activity [IDA]annealing helicase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

KPNA4