BAIT

RPT1

CIM5, YTA3, proteasome regulatory particle base subunit RPT1, L000002557, YKL145W

ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance

UPS Project

GO Process (4)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IMP]

positive regulation of protein catabolic process [IDA]

proteasome regulatory particle assembly [IMP]

ubiquitin-dependent protein catabolic process [IPI]

Gene Ontology Molecular Function

ATPase activity [ISS]

Gene Ontology Cellular Component

proteasome regulatory particle, base subcomplex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

HSM3

L000004729, YBR272C

Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation

UPS Project

GO Process (2)

GO Function (0)

GO Component (4)

Gene Ontology Biological Process

mismatch repair [IMP]

proteasome regulatory particle assembly [IGI, IMP]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytosol [IDA]

nucleus [IDA]

proteasome regulatory particle, base subcomplex [IDA, IPI]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.1687 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: rpt1-1 - hsm3 [SGA score = -0.1687, P-value = 4.131E-18]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RPT1 HSM3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Funakoshi M (2009)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Funakoshi M (2009)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	8	BioGRID	3614654
RPT1 HSM3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Park S (2009)	Low	-	BioGRID	-
RPT1 HSM3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Sekaran S (2023)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Le Tallec B (2009)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Nahar A (2019)	Low	-	BioGRID	2650317
HSM3 RPT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Nahar A (2022)	Low	-	BioGRID	-
RPT1 HSM3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Park S (2009)	Low	-	BioGRID	335506
RPT1 HSM3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Park S (2013)	Low	-	BioGRID	-
RPT1 HSM3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Roelofs J (2009)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Roelofs J (2009)	Low	-	BioGRID	-
HSM3 RPT1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Saeki Y (2009)	Low	-	BioGRID	-
HSM3 RPT1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Takagi K (2012)	Low	-	BioGRID	-
HSM3 RPT1	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Barrault MB (2012)	Low	-	BioGRID	656906
RPT1 HSM3	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Le Tallec B (2009)	Low	-	BioGRID	341287
HSM3 RPT1	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Roelofs J (2009)	Low	-	BioGRID	335419
RPT1 HSM3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Bashore C (2015)	Low	-	BioGRID	-
HSM3 RPT1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Roelofs J (2009)	Low	-	BioGRID	-
HSM3 RPT1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Takagi K (2012)	Low	-	BioGRID	-
RPT1 HSM3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Takagi K (2012)	Low	-	BioGRID	-
HSM3 RPT1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Barrault MB (2012)	Low	-	BioGRID	656905
HSM3 RPT1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Le Tallec B (2009)	Low	-	BioGRID	-
HSM3 RPT1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Saeki Y (2009)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RPT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [ISS]

Gene Ontology Cellular Component

HSM3

Gene Ontology Biological Process

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By