BAIT

TOP1

MAK1, MAK17, DNA topoisomerase 1, L000002319, YOL006C
Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

"Superkiller" mutations suppress chromosomal mutations affecting double-stranded RNA killer plasmid replication in saccharomyces cerevisiae.

Toh-E A, Wickner RB

Saccharomyces cerevisiae strains carrying a 1.5 x 10(6)-dalton double-stranded RNA genome in virus-like particles (killer plasmid) secrete a protein toxin that kills strains not carrying this plasmid. At least 28 chromosomal genes (mak genes) are required to maintain or replicate this plasmid. Recessive mutations in any of four other chromosomal genes (ski for superkiller) result in enhanced toxin production. We ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 01, 1980; 77(1);527-30 [Pubmed: 6987655]

Throughput

  • Low Throughput

Ontology Terms

  • chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • ski4 suppresses mak1 mutation by retaining killer plasmid
  • ski4 suppresses mak17 mutation by retaining killer plasmid

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CSL4 TOP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4437BioGRID
2011658

Curated By

  • BioGRID