BAIT

CIS3

CCW11, CCW5, PIR4, SCW8, S000029072, L000003951, L000004553, S000029439, L000004569, YJL158C
Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
GO Process (1)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

HSP150

CCW7, ORE1, PIR2, heat shock protein HSP150, L000000824, L000001442, YJL159W
O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
GO Process (1)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Yeast cells harboring human alpha-1,3-fucosyltransferase at the cell surface engineered using Pir, a cell wall-anchored protein.

Abe H, Ohba M, Shimma Y, Jigami Y

Human alpha-1,3-fucosyltansferase (FucT) encoded by the FUT6 gene was displayed at the cell surface of yeast cells engineered using the yeast cell wall protein Pir1 or Pir2, and the FucT activity was detected at the surface of cells producing the Pir1-HA-FUT6 or Pir2-FLAG-FUT6 fusion proteins. To obtain higher activity, we engineered the host yeast cells in which endogenous PIR genes ... [more]

FEMS Yeast Res. Jan. 01, 2004; 4(4);417-25 [Pubmed: 14734022]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • Deletion of PIR4 causes synthetic lethality in a pir1 pir2 pir3 triple mutant grown on 0.01% SDS.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIS3 HSP150
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
161908

Curated By

  • BioGRID