TRAK2
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KCNJ2
Gene Ontology Biological Process
- cardiac muscle cell action potential involved in contraction [IMP]
- cellular potassium ion homeostasis [TAS]
- membrane depolarization during cardiac muscle cell action potential [TAS]
- membrane repolarization during action potential [IMP]
- membrane repolarization during cardiac muscle cell action potential [IMP, TAS]
- potassium ion import [IDA]
- potassium ion transmembrane transport [IDA]
- potassium ion transport [IDA]
- protein homotetramerization [IDA]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of membrane repolarization [IDA]
- regulation of resting membrane potential [TAS]
- regulation of skeletal muscle contraction via regulation of action potential [IMP]
- relaxation of cardiac muscle [IMP]
- relaxation of skeletal muscle [IMP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of gamma-aminobutyric acid receptor-interacting factor 1 (TRAK2) as a trafficking factor for the K+ channel Kir2.1.
To identify proteins that regulate potassium channel activity and expression, we performed functional screening of mammalian cDNA libraries in yeast that express the mammalian K(+) channel Kir2.1. Growth of Kir2.1-expressing yeast in media with low K(+) concentration is a function of K(+) uptake via Kir2.1 channels. Therefore, the host strain was transformed with a human cDNA library, and cDNA clones ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TRAK2 KCNJ2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID