Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes.

Jansa P, Mason SW, Hoffmann-Rohrer U, Grummt I

Termination of transcription by RNA polymerase I (Pol I) is a two-step process which involves pausing of elongating transcription complexes and release of both pre-rRNA and Pol I from the template. In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. Dissociation of paused ... [more]

EMBO J. May. 15, 1998; 17(10);2855-64 [Pubmed: 9582279]

Throughput

  • Low Throughput

Curated By

  • BioGRID