BAIT

CAP2

F-actin-capping protein subunit beta, L000000215, YIL034C
Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
GO Process (2)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ACT1

ABY1, END7, actin, L000000025, YFL039C
Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

  • -0.5893 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
  • alleles: cap2 - act1-101 [SGA score = -0.5893, P-value = 6.301E-20]
  • alleles: cap2 - act1-105 [SGA score = -0.3812, P-value = 1.269E-10]
  • alleles: cap2 - act1-108 [SGA score = -0.3187, P-value = 5.909E-5]
  • alleles: cap2 - act1-119 [SGA score = -0.2682, P-value = 3.802E-13]
  • alleles: cap2 - act1-120 [SGA score = -0.2444, P-value = 0.01151]
  • alleles: cap2 - act1-121 [SGA score = -0.3345, P-value = 2.784E-7]
  • alleles: cap2 - act1-122 [SGA score = -0.3579, P-value = 1.54E-8]
  • alleles: cap2 - act1-124 [SGA score = -0.4845, P-value = 8.619E-9]
  • alleles: cap2 - act1-125 [SGA score = -0.3492, P-value = 8.224E-6]
  • alleles: cap2 - act1-129 [SGA score = -0.4476, P-value = 1.721E-13]
  • alleles: cap2 - act1-132 [SGA score = -0.3437, P-value = 2.211E-12]
  • alleles: cap2 - act1-155 [SGA score = -0.4759, P-value = 3.691E-14]
  • alleles: cap2 - act1-159 [SGA score = -0.2448, P-value = 4.423E-5]
  • alleles: cap2 - act1-3 [SGA score = -0.4050, P-value = 0]
  • alleles: cap2 - act1-4 [SGA score = -0.5053, P-value = 3.297E-25]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ACT1 CAP2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1777BioGRID
377029
ACT1 CAP2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.6969BioGRID
1979282
CAP2 ACT1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
161926

Curated By

  • BioGRID