BAIT
DNA2
WEB2, bifuctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2, L000003158, YHR164C
Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair and processing of meiotic DNA double strand breaks; required for normal life span; component of telomeric chromatin, with cell-cycle dependent localization; required for telomerase-dependent telomere synthesis; forms nuclear foci upon DNA replication stress
GO Process (7)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
SOD1
CRS4, superoxide dismutase SOD1, L000001978, YJR104C
Cytosolic copper-zinc superoxide dismutase; detoxifies superoxide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p and enters the nucleus under oxidative stress to promote transcription of stress response genes; human ortholog implicated in ALS; abundance increases under DNA replication stress and during exposure to boric acid; localization of a fraction to the mitochondrial intermembrane space is modulated by the MICOS complex
GO Process (10)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- age-dependent response to reactive oxygen species involved in chronological cell aging [IMP]
- cellular copper ion homeostasis [IMP]
- cellular zinc ion homeostasis [IMP]
- fungal-type cell wall organization [IMP]
- negative regulation of cellular respiration [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress [IMP]
- protein stabilization [IMP, IPI]
- removal of superoxide radicals [IBA]
- superoxide metabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
A network of multi-tasking proteins at the DNA replication fork preserves genome stability.
To elucidate the network that maintains high fidelity genome replication, we have introduced two conditional mutant alleles of DNA2, an essential DNA replication gene, into each of the approximately 4,700 viable yeast deletion mutants and determined the fitness of the double mutants. Fifty-six DNA2-interacting genes were identified. Clustering analysis of genomic synthetic lethality profiles of each of 43 of the ... [more]
PLoS Genet. Dec. 01, 2005; 1(6);e61 [Pubmed: 16327883]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID