BAIT

MYO5

myosin 5, L000002935, YMR109W
One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

ACT1

ABY1, END7, actin, L000000025, YFL039C
Actin; structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

  • -0.5421 [SGA Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
  • alleles: myo5 - act1-101 [SGA score = -0.3433, P-value = 2.698E-38]
  • alleles: myo5 - act1-105 [SGA score = -0.1477, P-value = 0.007746]
  • alleles: myo5 - act1-108 [SGA score = -0.3429, P-value = 5.012E-5]
  • alleles: myo5 - act1-119 [SGA score = -0.1662, P-value = 0.0003852]
  • alleles: myo5 - act1-121 [SGA score = -0.4058, P-value = 1.29E-12]
  • alleles: myo5 - act1-122 [SGA score = -0.4197, P-value = 7.691E-71]
  • alleles: myo5 - act1-124 [SGA score = -0.2600, P-value = 2.826E-5]
  • alleles: myo5 - act1-125 [SGA score = -0.2365, P-value = 0.01051]
  • alleles: myo5 - act1-129 [SGA score = -0.3185, P-value = 0.01604]
  • alleles: myo5 - act1-132 [SGA score = -0.3346, P-value = 0.000153]
  • alleles: myo5 - act1-136 [SGA score = -0.4160, P-value = 1.22E-6]
  • alleles: myo5 - act1-155 [SGA score = -0.2497, P-value = 0.002094]
  • alleles: myo5 - act1-159 [SGA score = -0.4560, P-value = 1.294E-17]
  • alleles: myo5 - act1-3 [SGA score = -0.1466, P-value = 0.02591]
  • alleles: myo5 - act1-4 [SGA score = -0.5421, P-value = 1.754E-25]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MYO5 ACT1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
MYO5 ACT1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
ACT1 MYO5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2459BioGRID
377000
ACT1 MYO5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3956BioGRID
1979437
ACT1 MYO5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID