BNI1
Gene Ontology Biological Process
- actin filament bundle assembly [IGI, IMP]
- actin nucleation [IDA]
- actomyosin contractile ring actin filament bundle assembly [IMP]
- barbed-end actin filament capping [IDA]
- budding cell apical bud growth [IGI, IMP]
- establishment of mitotic spindle orientation [IMP]
- formin-nucleated actin cable assembly [IDA, IGI, IMP]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- regulation of protein localization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ACT1
Gene Ontology Biological Process
- DNA repair [IDA]
- actomyosin contractile ring contraction [IDA, IMP]
- ascospore wall assembly [IDA]
- budding cell isotropic bud growth [TAS]
- cellular response to oxidative stress [IGI]
- chronological cell aging [IMP]
- endocytosis [IMP]
- establishment of cell polarity [IGI]
- establishment of mitotic spindle orientation [TAS]
- exocytosis [TAS]
- fungal-type cell wall organization [TAS]
- histone acetylation [IDA]
- mitochondrion inheritance [TAS]
- protein secretion [IGI, IMP]
- vacuole inheritance [IGI, IMP]
- vesicle transport along actin filament [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
A global genetic interaction network maps a wiring diagram of cellular function.
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]
Quantitative Score
- -0.5002 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
- alleles: bni1 - act1-101 [SGA score = -0.5002, P-value = 3.833E-20]
- alleles: bni1 - act1-108 [SGA score = -0.1288, P-value = 0.001043]
- alleles: bni1 - act1-119 [SGA score = -0.2114, P-value = 0.0004451]
- alleles: bni1 - act1-120 [SGA score = -0.2194, P-value = 0.01051]
- alleles: bni1 - act1-121 [SGA score = -0.4180, P-value = 2.634E-13]
- alleles: bni1 - act1-124 [SGA score = -0.2768, P-value = 0.00038]
- alleles: bni1 - act1-125 [SGA score = -0.2090, P-value = 4.166E-11]
- alleles: bni1 - act1-132 [SGA score = -0.2871, P-value = 0.001241]
- alleles: bni1 - act1-136 [SGA score = -0.3062, P-value = 9.752E-8]
- alleles: bni1 - act1-155 [SGA score = -0.3298, P-value = 9.518E-69]
- alleles: bni1 - act1-3 [SGA score = -0.3029, P-value = 0.000135]
- alleles: bni1 - act1-4 [SGA score = -0.2596, P-value = 5.394E-11]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BNI1 ACT1 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 617535 | |
| BNI1 ACT1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 196305 | |
| BNI1 ACT1 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 196299 | |
| ACT1 BNI1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| BNI1 ACT1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | High | - | BioGRID | 1238617 | |
| BNI1 ACT1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 196301 | |
| BNI1 ACT1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID