BAIT

UGA3

L000002423, YDL170W

Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4

GO Process (3)

GO Function (4)

GO Component (1)

Gene Ontology Biological Process

gamma-aminobutyric acid catabolic process [IMP]

nitrogen catabolite activation of transcription from RNA polymerase II promoter [IMP]

nitrogen utilization [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]
sequence-specific DNA binding transcription factor recruiting transcription factor activity [IDA, IGI]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

DLT1

YMR126C

Protein of unknown function; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.3026 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: uga3 - dlt1 [SGA score = -0.3026, P-value = 0.0003322]

Curated By

BioGRID

Interaction Summary