BAIT

HAC1

ERN4, IRE15, transcription factor HAC1, L000002611, YFL031W

Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress

GO Process (4)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

endoplasmic reticulum unfolded protein response [IMP]

negative regulation of transcription from RNA polymerase II promoter during meiosis [IDA, IMP]

positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]

positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response [IMP]

Gene Ontology Molecular Function

sequence-specific DNA binding [IDA]
sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RPN4

SON1, UFD5, stress-regulated transcription factor RPN4, L000001984, YDL020C

Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

GO Process (7)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription from RNA polymerase II promoter in response to stress [IMP]

positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IGI, IMP]

positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP]

positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance [IMP]

positive regulation of transcription from RNA polymerase II promoter in response to stress [IEP, IMP]

regulation of DNA repair [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.6428 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: hac1 - rpn4 [SGA score = -0.6428, P-value = 8.066E-54]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HAC1 RPN4	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Schmidt RM (2019)	Low	-	BioGRID	2542178
HAC1 RPN4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Bandyopadhyay S (2010)	High	-4.338	BioGRID	541661
RPN4 HAC1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.6458	BioGRID	364735
HAC1 RPN4	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.6458	BioGRID	377287
RPN4 HAC1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3347	BioGRID	2088814
RPN4 HAC1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Decourty L (2021)	High	-	BioGRID	3395419
RPN4 HAC1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Zheng J (2010)	High	-13.1264	BioGRID	508934
HAC1 RPN4	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Bircham PW (2011)	High	-	BioGRID	575678
RPN4 HAC1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Pan X (2006)	High	-	BioGRID	454535
HAC1 RPN4	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Schmidt RM (2019)	Low	-	BioGRID	2542186

Curated By

BioGRID

Interaction Summary

HAC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

sequence-specific DNA binding [IDA]sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

RPN4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

sequence-specific DNA binding [IDA]
sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, IMP]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
sequence-specific DNA binding [IDA]