BAIT

UBC7

DER2, QRI8, E2 ubiquitin-conjugating protein UBC7, L000001552, YMR022W
Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

HMG1

hydroxymethylglutaryl-CoA reductase (NADPH) HMG1, L000000789, YML075C
HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress; HMG1 has a paralog, HMG2, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Endoplasmic reticulum-associated degradation is required for cold adaptation and regulation of sterol biosynthesis in the yeast Saccharomyces cerevisiae.

Loertscher J, Larson LL, Matson CK, Parrish ML, Felthauser A, Sturm A, Tachibana C, Bard M, Wright R

Endoplasmic reticulum-associated degradation (ERAD) mediates the turnover of short-lived and misfolded proteins in the ER membrane or lumen. In spite of its important role, only subtle growth phenotypes have been associated with defects in ERAD. We have discovered that the ERAD proteins Ubc7 (Qri8), Cue1, and Doa10 (Ssm4) are required for growth of yeast that express high levels of the ... [more]

Eukaryotic Cell Apr. 01, 2006; 5(4);712-22 [Pubmed: 16607018]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cold sensitivity (APO:0000148)
  • phenotype: inviable (APO:0000112)

Curated By

  • BioGRID