BAIT
GET1
MDM39, YGL020C
Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
GO Process (2)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
PAF1
L000002621, YBR279W
Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
GO Process (25)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- chromatin organization involved in regulation of transcription [IMP]
- chromatin silencing at rDNA [IMP]
- global genome nucleotide-excision repair [IMP]
- mRNA 3'-end processing [IMP]
- negative regulation of DNA recombination [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of histone H3-K36 trimethylation [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- rRNA processing [IMP]
- regulation of chromatin silencing at telomere [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA]
- regulation of histone H2B ubiquitination [IMP]
- regulation of histone H3-K4 methylation [IMP]
- regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- snoRNA 3'-end processing [IMP]
- snoRNA transcription from an RNA polymerase II promoter [IDA, IMP]
- transcription elongation from RNA polymerase I promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
- transcription from RNA polymerase I promoter [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map.
Defining the functional relationships between proteins is critical for understanding virtually all aspects of cell biology. Large-scale identification of protein complexes has provided one important step towards this goal; however, even knowledge of the stoichiometry, affinity and lifetime of every protein-protein interaction would not reveal the functional relationships between and within such complexes. Genetic interactions can provide functional information that ... [more]
Nature Apr. 12, 2007; 446(7137);806-10 [Pubmed: 17314980]
Quantitative Score
- -12.024181 [SGA Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- An Epistatic MiniArray Profile (E-MAP) analysis was used to quantitatively score genetic interactions based on fitness defects estimated from the colony size of double versus single mutants. Genetic interactions were considered significant if they had an S score > 2.5 for positive interactions (suppression) and S score < -2.5 for negative interactions (synthetic sick/lethality).
Curated By
- BioGRID