BAIT

APN1

DNA-(apurinic or apyrimidinic site) lyase APN1, L000000096, YKL114C

Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity

GO Process (1)

GO Function (4)

GO Component (2)

Gene Ontology Biological Process

base-excision repair [IDA]

Gene Ontology Molecular Function

3'-tyrosyl-DNA phosphodiesterase activity [IDA]
DNA-(apurinic or apyrimidinic site) lyase activity [IDA, IMP, ISS]
double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]
phosphoric diester hydrolase activity [IDA]

Gene Ontology Cellular Component

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

APN2

ETH1, DNA-(apurinic or apyrimidinic site) lyase APN2, L000004434, YBL019W

Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII

GO Process (1)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

base-excision repair [IGI]

Gene Ontology Molecular Function

DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]
phosphoric diester hydrolase activity [IDA]

Gene Ontology Cellular Component

nucleus [IC]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.1349 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: apn1 - apn2 [SGA score = -0.1349, P-value = 0.001081]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Bennett RA (1999)	Low	-	BioGRID	162572
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Boiteux S (2006)	Low	-	BioGRID	205192
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Collura A (2012)	Low	-	BioGRID	617490
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Hanna M (2004)	Low	-	BioGRID	156070
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Hoopes JI (2017)	Low	-	BioGRID	2339758
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Johnson RE (1998)	Low	-	BioGRID	156076
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Stokdyk K (2021)	Low	-	BioGRID	3559617
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Xiao W (2001)	Low	-	BioGRID	426500
APN1 APN2	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Yu SL (2003)	Low	-	BioGRID	239841
APN1 APN2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Karumbati AS (2003)	Low	-	BioGRID	644007
APN1 APN2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Liu C (2002)	Low	-	BioGRID	426678
APN1 APN2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Manikova D (2010)	Low	-	BioGRID	353324
APN1 APN2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Guillet M (2002)	Low	-	BioGRID	157605
APN1 APN2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Guillet M (2003)	Low	-	BioGRID	157604
APN1 APN2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Maclean MJ (2003)	Low	-	BioGRID	157606
APN1 APN2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Matuo R (2010)	Low	-	BioGRID	342833
APN1 APN2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Xiao W (2001)	Low	-	BioGRID	426496

Curated By

BioGRID

Interaction Summary

APN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-tyrosyl-DNA phosphodiesterase activity [IDA]DNA-(apurinic or apyrimidinic site) lyase activity [IDA, IMP, ISS]double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]phosphoric diester hydrolase activity [IDA]

Gene Ontology Cellular Component

APN2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA-(apurinic or apyrimidinic site) lyase activity [IDA]double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]phosphoric diester hydrolase activity [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

3'-tyrosyl-DNA phosphodiesterase activity [IDA]
DNA-(apurinic or apyrimidinic site) lyase activity [IDA, IMP, ISS]
double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]
phosphoric diester hydrolase activity [IDA]

DNA-(apurinic or apyrimidinic site) lyase activity [IDA]
double-stranded DNA 3'-5' exodeoxyribonuclease activity [IDA]
phosphoric diester hydrolase activity [IDA]