BAIT

GAS1

CWH52, GGP1, 1,3-beta-glucanosyltransferase GAS1, L000000669, YMR307W

Beta-1,3-glucanosyltransferase; required for cell wall assembly and also has a role in transcriptional silencing; localizes to cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate previously unsuspected functions for Gas1 in DNA damage response and cell cycle regulation

GO Process (3)

GO Function (1)

GO Component (9)

Gene Ontology Biological Process

chromatin silencing [IMP, IPI]

filamentous growth [IMP]

fungal-type cell wall organization [IGI, IMP]

Gene Ontology Molecular Function

1,3-beta-glucanosyltransferase activity [IDA]

Gene Ontology Cellular Component

ER to Golgi transport vesicle [IDA]

cellular bud scar [IDA]

fungal-type cell wall [IDA]

integral component of membrane [ISM]

membrane raft [IDA]

mitochondrion [IDA]

nuclear periphery [IDA]

plasma membrane [IDA]

primary cell septum [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CWH41

DER7, GLS1, L000003121, L000000715, YGL027C

Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress

GO Process (2)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

fungal-type cell wall biogenesis [IMP]

protein N-linked glycosylation [IMP]

Gene Ontology Molecular Function

mannosyl-oligosaccharide glucosidase activity [IDA, IMP, ISS]

Gene Ontology Cellular Component

endoplasmic reticulum membrane [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C

We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to ... [more]

Science Sep. 23, 2016; 353(6306); [Pubmed: 27708008]

Quantitative Score

-0.5797 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Genetic interactions were considered significant if they had a p-value < 0.05 and an SGA score > 0.16 for positive interactions and SGA score < -0.12 for negative interactions.
alleles: gas1 - cwh41 [SGA score = -0.5797, P-value = 2.326E-38]

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CWH41 GAS1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.5045	BioGRID	381022
GAS1 CWH41	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.5045	BioGRID	406935
CWH41 GAS1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.6806	BioGRID	2114434
GAS1 CWH41	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Hoppins S (2011)	High	-9.3823	BioGRID	581885
GAS1 CWH41	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Schuldiner M (2005)	High	-6.9083	BioGRID	208187
CWH41 GAS1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Schuldiner M (2005)	High	-6.9083	BioGRID	207523
GAS1 CWH41	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Surma MA (2013)	High	-23.7707	BioGRID	898184
GAS1 CWH41	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Lesage G (2004)	High	-	BioGRID	485791
GAS1 CWH41	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	112477
CWH41 GAS1	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	112478

Curated By

BioGRID

Interaction Summary

GAS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

1,3-beta-glucanosyltransferase activity [IDA]

Gene Ontology Cellular Component

CWH41

Gene Ontology Biological Process

Gene Ontology Molecular Function

mannosyl-oligosaccharide glucosidase activity [IDA, IMP, ISS]

Gene Ontology Cellular Component

Negative Genetic

Publication

A global genetic interaction network maps a wiring diagram of cellular function.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By