An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Gene essentiality and synthetic lethality in haploid human cells.

Blomen VA, Majek P, Jae LT, Bigenzahn JW, Nieuwenhuis J, Staring J, Sacco R, van Diemen FR, Olk N, Stukalov A, Marceau C, Janssen H, Carette JE, Bennett KL, Colinge J, Superti-Furga G, Brummelkamp TR

Although the genes essential for life have been identified in less complex model organisms, their elucidation in human cells has been hindered by technical barriers. We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes required for optimal fitness under culture conditions. To study the principles of genetic interactions in human cells, we created a synthetic lethality ... [more]

Science Nov. 27, 2015; 350(6264);1092-6 [Pubmed: 26472760]

Throughput

Low Throughput

Additional Notes

High-confidence protein interaction

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CCT5 NLE1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9778	BioGRID	3058294

Curated By

BioGRID

Interaction Summary

NLE1

Gene Ontology Cellular Component

CCT5

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein beta-subunit binding [IPI]protein binding [IPI]