A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Gene essentiality and synthetic lethality in haploid human cells.

Blomen VA, Majek P, Jae LT, Bigenzahn JW, Nieuwenhuis J, Staring J, Sacco R, van Diemen FR, Olk N, Stukalov A, Marceau C, Janssen H, Carette JE, Bennett KL, Colinge J, Superti-Furga G, Brummelkamp TR

Although the genes essential for life have been identified in less complex model organisms, their elucidation in human cells has been hindered by technical barriers. We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes required for optimal fitness under culture conditions. To study the principles of genetic interactions in human cells, we created a synthetic lethality ... [more]

Science Nov. 27, 2015; 350(6264);1092-6 [Pubmed: 26472760]

Throughput

Low Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507)

Additional Notes

Aphenotypic negative genetic interaction (Synonym: Synthetic)
The authors note that their method cannot clearly distinguish between synthetic lethal or synthetic sick interactions (Figure 3B).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAB1A SEC23IP	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Han K (2017)	High	-3.156	BioGRID	2538758

Curated By

BioGRID

Interaction Summary

RAB1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

GDP binding [IBA]GTP binding [IBA]GTPase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SEC23IP