BAIT
DRD2
dopamine receptor D2
GO Process (99)
GO Function (11)
GO Component (15)
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IDA]
- G-protein coupled receptor signaling pathway [ISO]
- Wnt signaling pathway [IDA]
- acid secretion [IDA]
- activation of protein kinase activity [IDA]
- adenohypophysis development [ISO]
- adenylate cyclase-inhibiting dopamine receptor signaling pathway [IDA, ISO]
- adenylate cyclase-modulating G-protein coupled receptor signaling pathway [IDA]
- adult behavior [ISO]
- adult walking behavior [ISO]
- arachidonic acid secretion [ISO]
- associative learning [ISO]
- auditory behavior [IMP]
- axonogenesis [ISO]
- behavioral response to cocaine [ISO]
- behavioral response to ethanol [ISO]
- branching morphogenesis of a nerve [ISO]
- cellular potassium ion homeostasis [IC]
- cerebral cortex GABAergic interneuron migration [ISO]
- circadian regulation of gene expression [ISO]
- dopamine metabolic process [ISO]
- dopamine receptor signaling pathway [IDA, IMP, ISO, TAS]
- feeding behavior [ISO]
- forebrain development [IMP]
- grooming behavior [ISO]
- intracellular signal transduction [ISO]
- locomotory behavior [IMP, ISO]
- long-term memory [IMP]
- negative regulation of adenylate cyclase activity [IDA, ISO]
- negative regulation of blood pressure [ISO]
- negative regulation of cell migration [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of circadian sleep/wake cycle, sleep [IDA]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of dopamine receptor signaling pathway [ISO]
- negative regulation of dopamine secretion [IMP]
- negative regulation of insulin secretion [IMP]
- negative regulation of protein kinase B signaling [ISO]
- negative regulation of protein secretion [ISO]
- negative regulation of synaptic transmission, glutamatergic [ISO]
- negative regulation of voltage-gated calcium channel activity [IDA, ISO]
- neurological system process involved in regulation of systemic arterial blood pressure [ISO]
- neuron-neuron synaptic transmission [ISO]
- orbitofrontal cortex development [IEP]
- peristalsis [ISO]
- phosphatidylinositol metabolic process [IDA]
- phospholipase C-activating dopamine receptor signaling pathway [ISO]
- pigmentation [ISO]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of G-protein coupled receptor protein signaling pathway [IMP]
- positive regulation of cytokinesis [ISO]
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway [ISO]
- positive regulation of dopamine uptake involved in synaptic transmission [ISO]
- positive regulation of growth hormone secretion [ISO]
- positive regulation of long-term synaptic potentiation [IMP]
- positive regulation of mitosis [TAS]
- positive regulation of multicellular organism growth [ISO]
- positive regulation of neuroblast proliferation [ISO]
- positive regulation of receptor internalization [IMP]
- positive regulation of renal sodium excretion [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of urine volume [ISO]
- prepulse inhibition [ISO]
- protein localization [ISO]
- regulation of MAPK cascade [IDA]
- regulation of cAMP metabolic process [ISO]
- regulation of dopamine secretion [ISO]
- regulation of dopamine uptake involved in synaptic transmission [ISO]
- regulation of heart rate [ISO]
- regulation of locomotion involved in locomotory behavior [IMP]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of phosphoprotein phosphatase activity [IMP]
- regulation of potassium ion transport [IDA, ISO]
- regulation of sodium ion transport [ISO]
- regulation of synapse structural plasticity [IDA]
- regulation of synaptic transmission [IDA, ISO]
- regulation of synaptic transmission, GABAergic [ISO]
- release of sequestered calcium ion into cytosol [IMP]
- response to amphetamine [IEP, ISO]
- response to axon injury [IEP]
- response to cocaine [ISO]
- response to drug [IDA, ISO]
- response to ethanol [IEP]
- response to histamine [ISO]
- response to hypoxia [IMP]
- response to inactivity [IEP]
- response to iron ion [IEP]
- response to light stimulus [ISO]
- response to morphine [ISO]
- response to nicotine [IEP]
- response to toxic substance [IEP]
- sensory perception of smell [ISO]
- startle response [ISO]
- striatum development [IEP]
- synapse assembly [IEP]
- synaptic transmission [ISO]
- synaptic transmission, dopaminergic [IDA, IMP]
- temperature homeostasis [ISO]
- visual learning [ISO]
Gene Ontology Molecular Function- G-protein coupled receptor activity [ISO]
- dopamine binding [IMP, ISO]
- dopamine neurotransmitter receptor activity [IMP, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gi/Go [IDA, ISO]
- drug binding [IDA, ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
- G-protein coupled receptor activity [ISO]
- dopamine binding [IMP, ISO]
- dopamine neurotransmitter receptor activity [IMP, ISO]
- dopamine neurotransmitter receptor activity, coupled via Gi/Go [IDA, ISO]
- drug binding [IDA, ISO]
- identical protein binding [ISO]
- ionotropic glutamate receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
- acrosomal vesicle [IDA]
- axon [IDA, ISO]
- axon terminus [IDA]
- cytoplasmic vesicle [IDA]
- dendrite [IDA, ISO]
- dendritic spine [IDA]
- endocytic vesicle [IDA]
- integral component of plasma membrane [ISO]
- lateral plasma membrane [IDA]
- membrane [IDA, ISO]
- perikaryon [IDA]
- plasma membrane [IDA]
- postsynaptic density [IDA]
- sperm flagellum [IDA]
- synaptic vesicle membrane [IDA]
Rattus norvegicus
PREY
GRIA1
GluA1, gluR-A
glutamate receptor, ionotropic, AMPA 1
GO Process (30)
GO Function (16)
GO Component (26)
Gene Ontology Biological Process
- cellular response to amine stimulus [IEP]
- cellular response to amino acid stimulus [IEP]
- cellular response to dsRNA [IEP]
- cellular response to growth factor stimulus [IEP]
- cellular response to organic cyclic compound [IEP]
- cellular response to peptide hormone stimulus [IEP]
- ion transmembrane transport [IBA]
- ionotropic glutamate receptor signaling pathway [IDA, ISO]
- long term synaptic depression [ISO]
- long-term memory [IMP, ISO]
- neuronal action potential [IMP]
- positive regulation of membrane potential [IMP]
- positive regulation of synaptic transmission [IMP]
- receptor internalization [IDA, ISO]
- regulation of receptor recycling [IMP]
- regulation of synaptic plasticity [IMP, NAS]
- regulation of synaptic transmission [IDA]
- response to arsenic-containing substance [IEP]
- response to cocaine [IEP]
- response to drug [IEP]
- response to electrical stimulus [IEP]
- response to estradiol [IEP]
- response to fungicide [IEP]
- response to lithium ion [IEP]
- response to organic cyclic compound [IEP]
- response to peptide hormone [IEP]
- response to toxic substance [IMP]
- spinal cord development [IEP]
- synaptic transmission [ISO]
- synaptic transmission, glutamatergic [NAS]
Gene Ontology Molecular Function- G-protein alpha-subunit binding [IPI]
- G-protein beta-subunit binding [IDA]
- PDZ domain binding [IPI]
- adenylate cyclase binding [IDA]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IDA, ISO]
- beta-2 adrenergic receptor binding [IPI]
- extracellular-glutamate-gated ion channel activity [IBA]
- identical protein binding [IPI]
- ionotropic glutamate receptor activity [IDA]
- myosin V binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein homodimerization activity [TAS]
- protein kinase A binding [IDA]
- protein kinase binding [IPI]
- small GTPase binding [IPI]
- G-protein alpha-subunit binding [IPI]
- G-protein beta-subunit binding [IDA]
- PDZ domain binding [IPI]
- adenylate cyclase binding [IDA]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IDA, ISO]
- beta-2 adrenergic receptor binding [IPI]
- extracellular-glutamate-gated ion channel activity [IBA]
- identical protein binding [IPI]
- ionotropic glutamate receptor activity [IDA]
- myosin V binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein homodimerization activity [TAS]
- protein kinase A binding [IDA]
- protein kinase binding [IPI]
- small GTPase binding [IPI]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA, ISO]
- asymmetric synapse [IDA]
- axonal spine [ISO]
- cell surface [IDA, ISO]
- cytosol [IDA]
- dendrite [ISO, ISS]
- dendrite membrane [ISO]
- dendritic shaft [IDA]
- dendritic spine [IDA, ISO]
- dendritic spine membrane [ISO]
- early endosome [IDA]
- endoplasmic reticulum [ISO]
- excitatory synapse [IDA]
- ionotropic glutamate receptor complex [ISO, TAS]
- membrane [ISO]
- neuromuscular junction [IDA]
- neuron projection [IDA, ISO]
- neuron spine [IDA, ISO]
- neuronal cell body [IDA, ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IBA, ISO]
- protein complex [IDA]
- recycling endosome [ISO]
- synapse [IDA, IMP, ISO]
- synaptic vesicle [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Protein-protein coupling/uncoupling enables dopamine D2 receptor regulation of AMPA receptor-mediated excitotoxicity.
here is considerable evidence that dopamine D2 receptors can modulate AMPA receptor-mediated neurotoxicity. However, the molecular mechanism underlying this process remains essentially unclear. Here we report that D2 receptors inhibit AMPA-mediated neurotoxicity through two pathways: the activation of phosphoinositide-3 kinase (PI-3K) and downregulation of AMPA receptor plasma membrane expression, both involving a series of protein-protein coupling/uncoupling events. Agonist stimulation of ... [more]
J. Neurosci. Apr. 27, 2005; 25(17);4385-95 [Pubmed: 15858065]
Throughput
- Low Throughput
Curated By
- BioGRID