RPB3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDC73
Gene Ontology Biological Process
- mRNA 3'-end processing [IMP]
- negative regulation of DNA recombination [IMP]
- positive regulation of histone H3-K36 trimethylation [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- transcription elongation from RNA polymerase I promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Subgenic Pol II interactomes identify region-specific transcription elongation regulators.
Transcription, RNA processing, and chromatin-related factors all interact with RNA polymerase II (Pol II) to ensure proper timing and coordination of transcription and co-transcriptional processes. Many transcription elongation regulators must function simultaneously to coordinate these processes, yet few strategies exist to explore the complement of factors regulating specific stages of transcription. To this end, we developed a strategy to purify ... [more]
Throughput
- High Throughput
Additional Notes
- 5' PolII enriched factors
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPB3 CDC73 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3309362 | |
| CDC73 RPB3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CDC73 RPB3 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -11.82 | BioGRID | 2357881 | |
| RPB3 CDC73 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| CDC73 RPB3 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | 2334928 | |
| CDC73 RPB3 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | 0 | BioGRID | 822094 |
Curated By
- BioGRID