PSRC1
Gene Ontology Biological Process
- cellular process [IDA]
- microtubule bundle formation [IDA]
- mitotic metaphase plate congression [ISO]
- negative regulation of cell growth [IMP]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity [IMP]
- positive regulation of microtubule polymerization [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of mitotic spindle organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TUBA1A
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway.
We have previously identified mouse DDA3 as a p53-inducible gene. To explore the functional role of DDA3, we screened a mouse brain cDNA library by the yeast two-hybrid assay, and identified the microtubule plus-end binding protein EB3 as a DDA3-interacting protein. Binding of DDA3 to EB3 was verified by glutathione S-transferase (GST) pull-down assay and subcellular colocalization; co-immunoprecipitation further indicated ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID