BAIT
GRIA2
GluA2, GluR-K2, GluR2, gluR-B
glutamate receptor, ionotropic, AMPA 2
GO Process (11)
GO Function (10)
GO Component (23)
Gene Ontology Biological Process
- establishment of protein localization [IMP]
- ion transmembrane transport [IBA]
- ionotropic glutamate receptor signaling pathway [IBA, ISO, ISS]
- positive regulation of synaptic transmission [IMP]
- protein tetramerization [IDA]
- receptor internalization [IDA]
- regulation of receptor recycling [IMP]
- regulation of synaptic transmission, glutamatergic [IMP]
- response to fungicide [IEP]
- response to lithium ion [IEP]
- synaptic transmission [IDA, ISO]
Gene Ontology Molecular Function- PDZ domain binding [IDA, IPI]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IDA, TAS]
- calcium channel regulator activity [TAS]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- identical protein binding [IPI]
- ionotropic glutamate receptor activity [IDA, ISO, TAS]
- kainate selective glutamate receptor activity [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor activity [IDA]
- PDZ domain binding [IDA, IPI]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IDA, TAS]
- calcium channel regulator activity [TAS]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- identical protein binding [IPI]
- ionotropic glutamate receptor activity [IDA, ISO, TAS]
- kainate selective glutamate receptor activity [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor activity [IDA]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA, ISO]
- asymmetric synapse [IDA]
- cell surface [IDA]
- dendrite [IDA]
- dendrite cytoplasm [IDA]
- dendritic shaft [IDA]
- dendritic spine [IDA]
- endoplasmic reticulum [ISO]
- growth cone [IDA]
- integral component of plasma membrane [IDA]
- ionotropic glutamate receptor complex [TAS]
- membrane [ISO]
- neuron projection [ISO]
- neuronal cell body [IDA]
- perikaryon [IDA]
- postsynaptic density [IDA]
- postsynaptic membrane [IBA, ISO]
- presynaptic membrane [IDA]
- protein complex [IDA, IPI]
- synapse [IDA, ISO]
- synaptic vesicle [ISO]
- synaptic vesicle membrane [IDA]
- terminal bouton [IDA]
Rattus norvegicus
PREY
PIK3R1
PI3KA
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
GO Process (54)
GO Function (24)
GO Component (12)
Gene Ontology Biological Process
- B cell differentiation [ISO]
- NFAT protein import into nucleus [ISO]
- aging [IEP]
- cellular glucose homeostasis [ISO, ISS]
- cellular response to UV [ISO]
- cellular response to fatty acid [IEP]
- cellular response to insulin stimulus [IEP, ISO, ISS]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- glucose metabolic process [IMP]
- growth hormone receptor signaling pathway [ISO]
- insulin receptor signaling pathway [IMP, ISS]
- insulin-like growth factor receptor signaling pathway [IMP, ISO, ISS]
- intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of blood pressure [IMP]
- negative regulation of cell-cell adhesion [IMP]
- negative regulation of cell-matrix adhesion [ISO]
- negative regulation of heart rate [IMP]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of osteoclast differentiation [ISO]
- negative regulation of proteolysis [IMP]
- negative regulation of smooth muscle cell proliferation [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- phosphatidylinositol phosphorylation [ISS]
- phosphatidylinositol-3-phosphate biosynthetic process [IDA, TAS]
- positive regulation of RNA splicing [ISO, ISS]
- positive regulation of cell migration [IMP, ISO]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO, ISS]
- positive regulation of gene expression [IMP]
- positive regulation of myoblast differentiation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription factor import into nucleus [ISO, ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor production [ISO]
- protein phosphorylation [ISO]
- protein stabilization [ISO, ISS]
- regulation of phosphatidylinositol 3-kinase activity [IDA]
- response to amino acid [IEP]
- response to cAMP [IDA]
- response to dexamethasone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO, ISS]
- response to estradiol [IEP]
- response to ethanol [IEP]
- response to fatty acid [IEP]
- response to fructose [IEP]
- response to glucocorticoid [IDA]
- response to growth factor [IEP]
- response to insulin [IDA]
- response to iron(II) ion [IEP]
- response to nutrient [IEP]
- response to progesterone [IEP]
- response to testosterone [IEP]
- response to yeast [IEP]
Gene Ontology Molecular Function- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Protein-protein coupling/uncoupling enables dopamine D2 receptor regulation of AMPA receptor-mediated excitotoxicity.
here is considerable evidence that dopamine D2 receptors can modulate AMPA receptor-mediated neurotoxicity. However, the molecular mechanism underlying this process remains essentially unclear. Here we report that D2 receptors inhibit AMPA-mediated neurotoxicity through two pathways: the activation of phosphoinositide-3 kinase (PI-3K) and downregulation of AMPA receptor plasma membrane expression, both involving a series of protein-protein coupling/uncoupling events. Agonist stimulation of ... [more]
J. Neurosci. Apr. 27, 2005; 25(17);4385-95 [Pubmed: 15858065]
Throughput
- Low Throughput
Curated By
- BioGRID