An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Calcineurin B-Like Protein-Interacting Protein Kinase CIPK21 Regulates Osmotic and Salt Stress Responses in Arabidopsis.

Pandey GK, Kanwar P, Singh A, Steinhorst L, Pandey A, Yadav AK, Tokas I, Sanyal SK, Kim BG, Lee SC, Cheong YH, Kudla J, Luan S

The role of calcium-mediated signaling has been extensively studied in plant responses to abiotic stress signals. Calcineurin B-like proteins (CBLs) and CBL-interacting protein kinases (CIPKs) constitute a complex signaling network acting in diverse plant stress responses. Osmotic stress imposed by soil salinity and drought is a major abiotic stress that impedes plant growth and development and involves calcium-signaling processes. In ... [more]

Plant Physiol. Sep. 01, 2015; 169(1);780-92 [Pubmed: 26198257]

Throughput

Low Throughput

Additional Notes

BiFC assay

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CBL2 CIPK21	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Braun P (2011)	High	-	BioGRID	-
CBL2 CIPK21	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Pandey GK (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CBL2

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [ISS]protein binding [IPI]

Gene Ontology Cellular Component

CIPK21