BAIT

KDR

CD309, FLK1, VEGFR, VEGFR2
kinase insert domain receptor
GO Process (31)
GO Function (9)
GO Component (9)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Sck is expressed in endothelial cells and participates in vascular endothelial growth factor-induced signaling.

Ratcliffe KE, Tao Q, Yavuz B, Stoletov KV, Spring SC, Terman BI

Sck, a member of the Shc family of cell signaling proteins, has only been studied in neuronal cells, though previous studies have demonstrated its expression in tissues other than brain. Using RT-PCR and RNase protection assays, we detected Sck mRNA expression in endothelial cells, and Sck protein was detected by Western blotting using polyclonal and monoclonal antibodies targeting the Sck ... [more]

Oncogene Sep. 12, 2002; 21(41);6307-16 [Pubmed: 12214271]

Throughput

  • Low Throughput

Ontology Terms

  • huvec cell (BTO:0001949)

Curated By

  • BioGRID