BAIT

FLT1

FLT, FLT-1, VEGFR-1, VEGFR1
fms-related tyrosine kinase 1
GO Process (20)
GO Function (7)
GO Component (5)
Homo sapiens
PREY

SHC2

SCK, SHCB, SLI
SHC (Src homology 2 domain containing) transforming protein 2
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Sck interacts with KDR and Flt-1 via its SH2 domain.

Igarashi K, Shigeta K, Isohara T, Yamano T, Uno I

Vascular endothelial growth factor (VEGF) is one of the major angiogenesis regulators. It binds to its tyrosine kinase receptors, KDR and Flt-1. However, little is known about their downstream signal transduction properties. We screened human brain cDNA library using the yeast two-hybrid system with the KDR cytoplasmic region as bait to find KDR binding proteins. After 6.2 x 10(6) clones ... [more]

Biochem. Biophys. Res. Commun. Oct. 09, 1998; 251(1);77-82 [Pubmed: 9790910]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FLT1 SHC2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID