BAIT

PUP2

DOA5, proteasome core particle subunit alpha 5, L000001531, YGR253C
Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

PMP1

L000001453, YCR024C-A
Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner.

Samra N, Atir-Lande A, Pnueli L, Arava Y

mRNA binding proteins (RBPs) constitute 10-15% of the eukaryotic proteome and play important part in post-transcriptional regulation of gene expression. Due to the instability of RNA and the transient nature its interaction with RBPs, identification of novel RBPs is a significant challenge. Recently, a novel methodology for RBP purification and identification (termed RaPID) was presented, which allows high affinity purification ... [more]

BMC Mol. Biol. Sep. 24, 2015; 16(0);17 [Pubmed: 26404137]

Throughput

  • High Throughput

Additional Notes

  • PMP1 mRNA
  • RaPID procedure

Curated By

  • BioGRID