BAIT

FVE

ACG1, ATMSI4, F3P11.12, F3P11_12, MSI4, MULTICOPY SUPPRESSOR OF IRA1 4, NFC04, NFC4, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR GROUP C, WD-40 REPEAT PROTEIN MSI4, AT2G19520
WD-40 repeat-containing protein MSI4
GO Process (6)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

HDA05

ATHDA5, HDA5, MAF19.7, MAF19_7, histone deacetylase 5, AT5G61060
histone deacetylase 5
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis.

Luo M, Tai R, Yu CW, Yang S, Chen CY, Lin WD, Schmidt W, Wu K

The acetylation level of histones on lysine residues regulated by histone acetyltransferases and histone deacetylases plays an important but under-studied role in the control of gene expression in plants. With the aim of characterizing the Arabidopsis RPD3/HDA1 family histone deacetylase HDA5, we present evidence showing that HDA5 displays deacetylase activity. Mutants defective in the expression of HDA5 displayed a late-flowering ... [more]

Plant J. Jun. 01, 2015; 82(6);925-36 [Pubmed: 25922987]

Throughput

  • Low Throughput

Additional Notes

  • BiFC assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HDA05 FVE
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
2204942
FVE HDA05
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID