BAIT
PARK2
Park, Prkn
parkin RBR E3 ubiquitin protein ligase
GO Process (63)
GO Function (22)
GO Component (22)
Gene Ontology Biological Process
- adult locomotory behavior [ISO, ISS]
- aggresome assembly [ISO, ISS]
- cellular protein catabolic process [ISO]
- cellular protein modification process [TAS]
- cellular response to toxic substance [ISO, ISS]
- dopamine metabolic process [ISO]
- dopamine uptake involved in synaptic transmission [ISO]
- learning [ISO]
- locomotory behavior [ISO]
- mitochondrial fission [ISS]
- mitochondrion degradation [ISO, ISS]
- mitochondrion organization [ISS]
- negative regulation of JNK cascade [ISS]
- negative regulation of actin filament bundle assembly [ISO]
- negative regulation of cell death [ISO]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of glucokinase activity [ISO, ISS]
- negative regulation of insulin secretion [ISO, ISS]
- negative regulation of mitochondrial fusion [ISS]
- negative regulation of neuron apoptotic process [ISO, ISS]
- negative regulation of neuron death [IGI, ISO]
- negative regulation of oxidative stress-induced neuron death [IGI]
- negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway [ISO, ISS]
- negative regulation of protein phosphorylation [ISO, ISS]
- negative regulation of reactive oxygen species metabolic process [ISO, ISS]
- negative regulation of release of cytochrome c from mitochondria [ISO, ISS]
- neuron cellular homeostasis [ISS]
- neuron maturation [IEP]
- norepinephrine metabolic process [ISO]
- positive regulation of DNA binding [ISO, ISS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO, ISS]
- positive regulation of mitochondrial fission [ISS]
- positive regulation of mitochondrial fusion [ISO, ISS]
- positive regulation of proteasomal protein catabolic process [ISO]
- positive regulation of protein linear polyubiquitination [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor-mediated signaling pathway [ISO, ISS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISO, ISS]
- protein K48-linked ubiquitination [ISO, ISS]
- protein K63-linked ubiquitination [ISO, ISS]
- protein autoubiquitination [ISO, ISS]
- protein localization to mitochondrion [ISS]
- protein metabolic process [ISO]
- protein monoubiquitination [ISO, ISS]
- protein polyubiquitination [ISO, ISS]
- protein ubiquitination [ISO, ISS]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISO, ISS]
- regulation of autophagy [ISO, ISS]
- regulation of cellular response to oxidative stress [ISS]
- regulation of mitochondrion organization [ISO, ISS]
- regulation of neurotransmitter secretion [ISO]
- regulation of reactive oxygen species metabolic process [ISO]
- regulation of synaptic transmission [ISO]
- response to corticosterone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO]
- response to oxidative stress [ISS]
- response to unfolded protein [IDA]
- startle response [ISO]
- synaptic transmission, dopaminergic [ISO]
- synaptic transmission, glutamatergic [ISO]
- ubiquitin-dependent protein catabolic process [TAS]
- zinc ion homeostasis [ISS]
Gene Ontology Molecular Function- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
- F-box domain binding [ISO, ISS]
- G-protein coupled receptor binding [ISO, ISS]
- Hsp70 protein binding [ISO, ISS]
- PDZ domain binding [ISO, ISS]
- actin binding [ISO]
- chaperone binding [ISO]
- cullin family protein binding [ISO, ISS]
- heat shock protein binding [ISO]
- histone deacetylase binding [ISO]
- identical protein binding [ISO, ISS]
- kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [IDA]
- protein kinase binding [ISO, ISS]
- transcription regulatory region sequence-specific DNA binding [ISS]
- tubulin binding [ISO]
- ubiquitin binding [ISO, ISS]
- ubiquitin conjugating enzyme binding [ISO, ISS]
- ubiquitin protein ligase activity [ISO]
- ubiquitin protein ligase binding [ISO]
- ubiquitin-protein transferase activity [ISO, ISS, TAS]
- ubiquitin-specific protease binding [ISO]
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- Golgi membrane [IDA]
- LUBAC complex [ISO, ISS]
- Parkin-FBXW7-Cul1 ubiquitin ligase complex [ISO, ISS]
- SCF ubiquitin ligase complex [ISO]
- aggresome [ISO, ISS]
- cytoplasm [IDA, ISO, ISS]
- cytoplasmic vesicle [IDA]
- cytosol [ISO, ISS]
- endoplasmic reticulum [IDA, ISO]
- endoplasmic reticulum membrane [IDA]
- intracellular [IDA]
- membrane raft [IDA]
- mitochondrion [ISO, ISS]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [IDA]
- protein complex [ISO]
- synapse [IDA]
- synaptic vesicle [IDA]
- ubiquitin ligase complex [ISO, ISS]
Rattus norvegicus
PREY
TRIB3
NIPK
tribbles pseudokinase 3
GO Process (13)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- cellular response to insulin stimulus [ISO]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO, ISS]
- negative regulation of fat cell differentiation [ISO]
- negative regulation of fatty acid biosynthetic process [ISO]
- negative regulation of nucleic acid-templated transcription [ISO]
- negative regulation of protein kinase activity [ISO, ISS]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of protein binding [ISO]
- positive regulation of ubiquitin-protein transferase activity [ISO]
- programmed cell death [NAS]
- regulation of MAP kinase activity [ISO, ISS]
- regulation of glucose transport [ISO]
- response to endoplasmic reticulum stress [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Trib3 Is Elevated in Parkinson's Disease and Mediates Death in Parkinson's Disease Models.
Parkinson's disease (PD) is characterized by the progressive loss of select neuronal populations, but the prodeath genes mediating the neurodegenerative processes remain to be fully elucidated. Trib3 (tribbles pseudokinase 3) is a stress-induced gene with proapoptotic activity that was previously described as highly activated at the transcriptional level in a 6-hydroxydopamine (6-OHDA) cellular model of PD. Here, we report that ... [more]
J. Neurosci. Jul. 29, 2015; 35(30);10731-49 [Pubmed: 26224857]
Throughput
- Low Throughput
Curated By
- BioGRID