An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HPF1/C4orf27 Is a PARP-1-Interacting Protein that Regulates PARP-1 ADP-Ribosylation Activity.

Gibbs-Seymour I, Fontana P, Rack JG, Ahel I

We report the identification of histone PARylation factor 1 (HPF1; also known as C4orf27) as a regulator of ADP-ribosylation signaling in the DNA damage response. HPF1/C4orf27 forms a robust protein complex with PARP-1 in cells and is recruited to DNA lesions in a PARP-1-dependent manner, but independently of PARP-1 catalytic ADP-ribosylation activity. Functionally, HPF1 promotes PARP-1-dependent in trans ADP-ribosylation of ... [more]

Mol. Cell May. 05, 2016; 62(3);432-42 [Pubmed: 27067600]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HIST2H2AC APLF	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9994	BioGRID	3074671

Curated By

BioGRID

Interaction Summary

APLF

Gene Ontology Biological Process

Gene Ontology Molecular Function

3'-5' exonuclease activity [IDA]DNA-(apurinic or apyrimidinic site) lyase activity [IDA]endodeoxyribonuclease activity [IDA]nucleotide binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HIST2H2AC