An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Serine ADP-Ribosylation Depends on HPF1.

Bonfiglio JJ, Fontana P, Zhang Q, Colby T, Gibbs-Seymour I, Atanassov I, Bartlett E, Zaja R, Ahel I, Matic I

ADP-ribosylation (ADPr) regulates important patho-physiological processes through its attachment to different amino acids in proteins. Recently, by precision mapping on all possible amino acid residues, we identified histone serine ADPr marks in the DNA damage response. However, the biochemical basis underlying this serine modification remained unknown. Here we report that serine ADPr is strictly dependent on histone PARylation factor 1 ... [more]

Mol. Cell Feb. 08, 2017; 0(0); [Pubmed: 28190768]

Throughput

Low Throughput

Additional Notes

Serine ADP-Ribosylation
reaction requires the presence of HPF1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
H3F3A PARP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Drane P (2010)	Low	-	BioGRID	-
H3F3A PARP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Heo K (2007)	Low	-	BioGRID	-
H3F3A PARP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Drane P (2010)	Low	-	BioGRID	-
H3F3A PARP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Siddaway R (2022)	High	-	BioGRID	3511812
H3F3A PARP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Messner S (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PARP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD+ ADP-ribosyltransferase activity [IDA, TAS]enzyme binding [IPI]identical protein binding [IPI]poly(A) RNA binding [IDA]protein N-terminus binding [IPI]protein binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

H3F3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding [IDA]RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]nucleosomal DNA binding [IDA]protein binding [IPI]